Recombinant Streptavidin
| 【Numbering】BK-NRPA05S | 【CAS】CAS | ||
| 【Item No.】BK-NRPA05S-100 | 【specification】 | ||
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Name: Recombinant Streptavidin
Specifications: 1 mg, 10 mg, 100 mg, 500 mg
Product form: lyophilized powder, the solution before lyophilization is 5mM PB (4mM Na2HPO4, 1mM NaH2PO4, pH7.4) solution
Molecular weight: 16.7 kDa
Isoelectric point: 7.95
Purity: ≥95% (SDS-PAGE detection)
Concentration determination: 280nm absorption method, molar absorption coefficient is 4.19×104(mol/L)-1·cm-1, that is, c(g/L) = A280nm/2.51
Endotoxin: ≤5EU/mg
Source: Escherichia coli
Storage conditions: -20℃ storage
Validity period: 3 years
Streptavidin (SA) has a high affinity with biotin, and the affinity constant Kd can generally reach 10-15M, and active SA usually exists in the form of tetramers. Because SA does not contain sugar groups, SA has a lower non-specific binding level than avidin in detection applications. It has been widely used in enzyme-linked immunosorbent assays, immunohistochemistry, time-resolved immunofluorescence (TRFIA), Quantitative PCR, single-stranded DNA preparation, biomolecule purification, monoclonal antibody preparation and other biotechnological fields.
However, the super-high affinity of SA and biotin is generally difficult to separate the combined SA from biotin by a gentle method. The conditions that can separate will generally make SA inactive, and the same biotin-labeled antibodies and antigens will also lose activity. Therefore, it is generally believed that the binding of SA and biotin is irreversible. Recombinant streptavidin 2 (r-SA2) is modified at the molecular level on the basis of natural streptavidin to eliminate the binding force to form tetramers. When it is active, it is a monomer, and at the same time greatly weakens the biological The affinity (Kd≈10-7M) of biotin can realize reversible binding and dissociation with biotin, and can be used for gentle separation, purification and recycling of biotinylated proteins, antibodies, nucleic acids and other substances.
M: protein molecular weight standard
Lane 1: r-SA2
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Specifications: 1 mg, 10 mg, 100 mg, 500 mg
Product form: lyophilized powder, the solution before lyophilization is 5mM PB (4mM Na2HPO4, 1mM NaH2PO4, pH7.4) solution
Molecular weight: 16.7 kDa
Isoelectric point: 7.95
Purity: ≥95% (SDS-PAGE detection)
Concentration determination: 280nm absorption method, molar absorption coefficient is 4.19×104(mol/L)-1·cm-1, that is, c(g/L) = A280nm/2.51
Endotoxin: ≤5EU/mg
Source: Escherichia coli
Storage conditions: -20℃ storage
Validity period: 3 years
Streptavidin (SA) has a high affinity with biotin, and the affinity constant Kd can generally reach 10-15M, and active SA usually exists in the form of tetramers. Because SA does not contain sugar groups, SA has a lower non-specific binding level than avidin in detection applications. It has been widely used in enzyme-linked immunosorbent assays, immunohistochemistry, time-resolved immunofluorescence (TRFIA), Quantitative PCR, single-stranded DNA preparation, biomolecule purification, monoclonal antibody preparation and other biotechnological fields.
However, the super-high affinity of SA and biotin is generally difficult to separate the combined SA from biotin by a gentle method. The conditions that can separate will generally make SA inactive, and the same biotin-labeled antibodies and antigens will also lose activity. Therefore, it is generally believed that the binding of SA and biotin is irreversible. Recombinant streptavidin 2 (r-SA2) is modified at the molecular level on the basis of natural streptavidin to eliminate the binding force to form tetramers. When it is active, it is a monomer, and at the same time greatly weakens the biological The affinity (Kd≈10-7M) of biotin can realize reversible binding and dissociation with biotin, and can be used for gentle separation, purification and recycling of biotinylated proteins, antibodies, nucleic acids and other substances.
M: protein molecular weight standard
Lane 1: r-SA2
| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance |
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| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance.The website pictures are from the Internet. The pictures are for reference only. Please take the real object as the standard. In case of infringement, please contact us to delete them immediately. |
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