DNA fragment screening
| 【Numbering】BK2021062316 | 【CAS】N/A | ||
| 【Item No.】BK2021062316-03 | 【specification】 | ||
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This fragment sorting kit is suitable for rapid sorting and recovery of DNA fragment sizes in the construction of high-throughput sequencing libraries (NGS). Disperse directly in the binding buffer, adjust the volume ratio of the magnetic bead suspension to the sample, and choose the left, right, or double-sided DNA fragments. The purified fragment does not contain contaminants such as nucleotides, primer dimers, linkers, enzymes, and salts. It is a next-generation sequencing workflow that provides an efficient magnetic bead-based purification system. It is a large-scale, high-throughput fragment sorting kit specially developed for supporting automated workbenches. It can be applied to almost all automated instruments or manually Perform fragment separation of a small number of samples.
■ Product performance
The eluted DNA is of high purity, the ratio of A260/A280 is between 1.7-1.9, and the ratio of A260/A230 is usually above 2.0. The recovery rate of magnetic beads is high, >90% over 500bp, >80% over 200bp, and >70% over 100bp.
■ Application direction
Rapid sorting and recovery of DNA fragment size in the construction of high-throughput sequencing library (NGS);
You can choose the left, right, or double-sided DNA fragments;
After 1.8 times the volume of the suspension is combined, washed and eluted, it can be used for the purification of PCR and enzyme reaction products.
■ Matters needing attention
1. The magnetic beads are stored in deionized water, freezing, drying and centrifugation may affect the use of magnetic beads;
2. It is normal for the magnetic beads to settle due to gravity. Before use, be sure to fully shake and mix the magnetic beads;
| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance |
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| Warm tips: Suzhou Beike nano products are only used for scientific research, not for human body,different batches of products have different specifications and performance.The website pictures are from the Internet. The pictures are for reference only. Please take the real object as the standard. In case of infringement, please contact us to delete them immediately. |
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